九龙牦牛肌细胞生成素基因的克隆及其表达谱

doi:10.11843/j.issn.0366-6964.2014.02.006

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (2): 207-211.doi: 10.11843/j.issn.0366-6964.2014.02.006

九龙牦牛肌细胞生成素基因的克隆及其表达谱

林亚秋^*，张倡珲，郑玉才，王永，黄林

（西南民族大学生命科学与技术学院，成都 610041）

收稿日期:2013-07-03 出版日期:2014-02-23 发布日期:2014-02-23
通讯作者: 林亚秋，E-mail：linyq1999@163.com
作者简介:林亚秋（1976-），女，内蒙古海拉尔人，博士，副教授，主要从事动物遗传育种研究
基金资助:
国家民委项目（12XNZ002）；西南民族大学中央高校基本科研业务费专项(13NZYQN23)

Cloning and Expression Profiling of Myogenin Gene of Jiulong Yak

LIN Ya-qiu^*，ZHANG Chang-hui，ZHENG Yu-cai，WANG Yong，HUANG Lin

(College of Life Science and Technology，Southwest University for Nationalities，Chengdu 610041, China)

Received:2013-07-03 Online:2014-02-23 Published:2014-02-23

摘要/Abstract

摘要：

旨在获得九龙牦牛（Bos grunniens）肌细胞生成素（Myogenin，MyoG）基因序列并进行生物信息学分析，同时分析其组织表达谱和时序表达谱，为进一步研究九龙牦牛肌肉发育和肉质形成奠定基础。本试验通过RT-PCR技术克隆九龙牦牛MyoG基因，利用半定量RT-PCR技术检测该基因在九龙牦牛不同组织中的表达情况，利用荧光定量PCR技术检测该基因在九龙牦牛不同发育时期背最长肌的表达情况。结果表明，获得九龙牦牛700 bp的MyoG基因序列（GenBank登录号：KC184120），其中ORF为675 bp，编码224个氨基酸，与普通牛、人、小鼠、大鼠、猪、羊和兔MyoG氨基酸序列同源性为92%～99%；MyoG基因仅在背最长肌中检测到表达，在心、肝、脾、肾和脂肪中未检测到表达；MyoG基因随着九龙牦牛年龄段增长表达呈上升趋势，且在3.5～5.5和9岁以上的牛肌背最长肌中的表达显著高于0.5岁牛的表达水平（P<0.05）。结果表明，MyoG基因可能在九龙牦牛肌肉组织发育过程中发挥重要的调控作用。

Abstract:

The aim of the present study was to clone myogenin (MyoG) gene of Jiulong yak，to carry out bioinformatics analysis and to assay gene expression profiling in different tissues and developmental stages，in order to lay the foundation for studing meat quality formation and muscle development of Jiulong yak.Based on the published nucleotide sequence of bovine MyoG gene in GenBank，a pair of primer was designed.The Jiulong yak MyoG gene was amplified by RT-PCR，the tissue specificity of MyoG gene were analyzed by semi-quantitative RT-PCR，and different development stages expression specificity of MyoG gene were analyzed by fluorescence quantitative PCR．The results showed that Jiulong yak MyoG gene cDNA was 700 bp (GenBank accession number：KC184120)，with an ORF of 675 bp encoding 224 amino acids.Compared with cattle，human，mouse，rat，pigs，sheep，rabbit，the homology of amino acid sequences were 92%-99%.The MyoG gene expression was only observed in longissimus muscle，and not detected in heart，liver，kidney，spleen and fat.The MyoG mRNA expression level increased with Jiulong yak growth，showing higher mRNA level in longissimus muscle of 3.5-5.5 and over 9 years old Jiulong yaks than that in 0.5 years old Jiulong yaks (P<0.05).These results suggest that MyoG may play an important role in the development of muscle tissue of Jiulong yak.

中图分类号:

林亚秋，张倡珲，郑玉才，王永，黄林. 九龙牦牛肌细胞生成素基因的克隆及其表达谱[J]. 畜牧兽医学报, 2014, 45(2): 207-211.

LIN Ya-qiu，ZHANG Chang-hui，ZHENG Yu-cai，WANG Yong，HUANG Lin. Cloning and Expression Profiling of Myogenin Gene of Jiulong Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 207-211.

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九龙牦牛肌细胞生成素基因的克隆及其表达谱

Cloning and Expression Profiling of Myogenin Gene of Jiulong Yak

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